Actin filaments were assembled in vitro and labeled/stabilized with rhodamine phalloidin. The filaments were sheared into short fragments before microinjection into NRK cells during early anaphase. The site of injection appeared as a large bright spot near the top of the image. During the early phase of cytokinesis, several small aggregates of actin filaments dissociated from the site of injection and moved toward the equator, which is marked by two arrows. Recording time, 6 min. |